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Quenchers
Black Hole Quenchers
BHQ-1® - BHQ-2® - BHQ-3®
We have been synthesizing custom oligonucleotides and qPCR probes labeled with the original BHQ® quencher dyes for decades. We produce and deliver your nucleic acid sequences for research, diagnostic and commercial use.
IP-free BHQ® quenchers for all applications
By purchasing your BHQ® labeled oligonucleotides and qPCR Probes from Eurogentec you can use them for diagnostic and commercial applications without license fees.
Unique Partner for all your applications
By ordering your BHQ® probes from us, you will benefit from our know-how, our experience and the quality of the original BHQ® quenchers. We are your unique partner all along your project, from your initial research and development phase to the commercial application.
BHQ-1®, BHQ-2® and BHQ-3®
We use the original Black Hole Quencher 1, 2 and 3 to label your oligonucleotides. BHQ® quenchers are the most popular and used quenchers for fluorescence quenched qPCR probes as they absorb a wide range of wavelengths from 480 nm to 730 nm.
| BHQ® Quenchers | Absorption Range (in nm) | IP Free D/Q pairs | Compatible fluorescent dyes |
|---|---|---|---|
| ● Black Hole Quencher 1 | 480 – 580 nm |
6-FAM/BHQ®-1 HEX/ BHQ®-1 Yakima Yellow/ BHQ®-1 |
ATTO 390 ATTO 425 ATTO 465 ATTO 488 ATTO 495 ATTO 520 ATTO 532 6-FAM HEX JOE Rhodamine-6G TET Yakima Yellow® |
| ● Black Hole Quencher 2 | 559 – 690 nm |
ROX™/BHQ®-2 ATTO 590/BHQ®-2 |
ATTO 550 ATTO 565 ATTO 590 ATTO 594 ATTO 610 Cy®3 Cy®5 DragonFly Orange ROX™ TAMRA Texas Red® |
| ● Black Hole Quencher 3 | 650 – 730 nm |
ATTO 620 ATTO 633 ATTO 637 ATTO 647N ATTO 655 ATTO 680 ATTO 700 |
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View the full IP-Free list
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Why choose BHQ® quenchers?
IP free for all applications
BHQ® patents for diagnostic and commercial use were lifted.
No native fluorescence
BHQ® were engineered to be true black quenchers with no native fluorescence.
Efficiently quenched fluorescent Dyes
BHQ® quench fluorescence with a very high signal-to-noise ratio.
Broad absorption range
BHQ® absorb fluorescent emission from 480 nm to 730 nm
Compatible with a large range of reporter dyes
BHQ® can be paired to a large panel of fluorescent dyes
Ideal for multiplexing assays
BHQ® have no residual background signal and a broad spectral range
How to order?
Usually, in dual-labeled qPCR Probes, Black Hole Quenchers are conjugated to the 3’end of the nucleic acid sequence. We can also modify your oligonucleotide at its 5’ terminus or at an internal position.
Diagnostic Grade Oligonucleotides
Pre-diagnostic and diagnostic grade oligonucleotides can be ordered after introducing a request.
Upon request you will be in contact with your dedicated account manager to discuss your project. After discussion and evaluation we will emit a quote for you.
Once we receive your agreement and the quote is signed, we will start the production of your nucleic acid sequences.
Other IP-Friendly Dyes
Our portfolio of Access™ fluorescent Dyes and Quenchers is comprehensive, spanning the full visible spectrum. We have D/Q pairs covering all the qPCR channels from Deep Blue to Infrared.