Catalog Oligonucleotides

Dye-labeled Calibration Oligos

Label

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What are calibration oligos?

Dye-labeled calibration oligos are 5’ fluorescent 10-mers dT oligonucleotides used as a reference to calibrate real-time qPCR thermocyclers.

 

Why use calibration oligos?

Calibration is a preliminary step meant to adjust spectral profiles for each single dye on the qPCR platforms. This allows to compensate for crosstalks between dyes (channels) in the context of multiplexed assays, which is critical for appropriate result analysis.

Specifications

Calibration Oligonucleotides are RP-HPLC purified, MS Quality controled and provided dried at a quantity of 5 nmol.

Oligo name Reference 5' Modification  Abs/Em (nm)
AP5-T10 UN-CT001-005 AP5 527/549 •
YY-T10 UN-CT005-005 Yakima Yellow® 530/549 •
HEX-T10 UN-CT010-005 HEX 535/556 •
DFO-T10 UN-CT015-005 Dragonfly Orange™ 554/576 •
TET-T10 UN-CT020-005 TET 521/536 •
JOE-T10 UN-CT025-005 JOE 529/555 •
HL647-T10 UN-CT030-005 Hilyte™ Fluor 647 650/675 •
ROX-T10 UN-CT035-005 ROX 575/602 •

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