IMG-EN-MD-NB085-04004-01.jpg
Oligos

3'-3' Terminus Linkage dA (inverted 3' base) - 1 modification

€33.00
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  • Cat.Number : MD-NB085-04004
  • Availability :
    In production

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Exonuclease activity, mainly at the 3’-terminus, is the primary cause of oligonucleotide degradation. The introduction at the 3’ terminus of 3’- deoxynucleosides bases, 2’, 3’-ddC or 3’ 3’-inverted base enhance resistance to degradation. These modifications also block the 3’-terminus from extension during polymerase extension (eg. PCR amplification) and allow cloning of specific alleles using 3’-blocked and 3’-OH oligonucleotides mixture for polymerase amplification of specific alleles.

Specifications

Chemistry
Molecular Mass/ Weight
  • 304,2
Modification
Modification Position
  • 3'
Storage & stability
Resuspension condition
  • - Spin the tube briefly to collect the pellet in the bottom of the tube<br>- Add an appropriate volume of recommended buffer (refer to technical data sheet)<br>- Allow the tube to stand a few minutes<br>- Vortex the tube for 15 seconds
Storage Conditions
  • Dried Oligonucleotides are stable for 18 months at ambiant temperature. <br>Oligonucleotides in solutions are stable for 24 months at -20°C. (Tolerance -20°C± 5°C. )<br> For more information please refer to the Reconstitution-Storage-Stability page<br>
Activity
Usage
  • Research use
Codes
Code Nacres
  • NA.51

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