Image of a kit SensoLyte 520 Cathepsin K Assay Kit Fluorimetric
Assay-kits

SensoLyte® 520 Cathepsin K Assay Kit Fluorimetric - 1 kit

€619.00
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  • Cat.Number : AS-72171
  • Availability :
    In production
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Cathepsins are a class of globular lysosomal proteases playing a vital role in mammalian cellular turnover. They degrade polypeptides and are distinguished by their substrate specificities. Cathepsin K is the lysosomal cysteine protease involved in bone remodeling and resorption. It has potential as a drug target in autoimmune diseases and osteoporosis.
The SensoLyte® 520 Cathepsin K Activity Assay Kit uses a QXL™ 520/HiLyte™ Fluor 488 FRET peptide substrate for the measurement of enzyme activity. In the intact FRET peptide, the fluorescence of HiLyte™ Fluor 488 is quenched by QXL™ 520. Upon cleavage of the FRET peptide by the active enzyme, the increase of fluorescence can be continuously monitored at excitation/emission = 490/520 nm. The fluorescent signal from HiLyte™ Fluor 488 is stable at low pH, which is optimal for cathepsin activity. The kit can be used to detect the activity of Cathepsin K enzyme in biological samples and purified enzyme preparations.

Specifications

Packaging
Kits components
  • Component A: QXL™ 520/HiLyte™ Fluor 488, Cathepsin K substrate, Ex/Em=490 nm/520 nm upon cleavage: 2 mM, 50 µL Component B: HiLyte™ Fluor 488, fluorescence reference standard, Ex/Em=490 nm/520 nm: 1 mM, 10 µL Component C: Procathepsin K, human recombinant: 0.15 mg/mL, 10 µL Component D: Assay Buffer: 20 mL Component E: Cathepsin K activation buffer: 100 µL Component F: Cathepsin K Inhibito:r 100 µM, 10 µL Component G: DTT: 1 M, 200 µL
Chemistry
UniProt number
  • P43235
Properties
Absorbance (nm)
  • 490
Emission (nm)
  • 520
Storage & stability
Storage Conditions
  • Store all kit components at -20°C, except for Component C. Store Component C at -80°C. Protect Components A and B from light and moisture. Component D can be stored at room temperature for convenience.
Activity
Application
Biomarker Target
Detection Method
Research Area
Sub-category Research Area
Usage
  • Research use
Codes
Code Nacres
  • NA.32

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