Image of a kit SensoLyte 520 IDE Activity Assay Kit Fluorimetric
Assay-kits

SensoLyte® 520 IDE Activity Assay Kit Fluorimetric - 1 kit

€619.00
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  • Cat.Number : AS-72231
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Insulin degrading enzyme (IDE) is considered a physiological and pathological relevant enzyme. Growing evidences have indicated that IDE may be involved in the pathogenesis of Alzheimer’s disease and diabetes mellitus type 2.
The SensoLyte® 520 IDE Activity Assay Kit can be used to detect enzyme activity in biological samples or in purified enzyme preparations. The unique FRET substrate was derived from an APP sequence designed to reduce cross reactivity with Neprilysin, ADAM10, TACE, BACE-1, and BACE-2. When active IDE cleaves the FRET substrate, it results in an increase of 5-FAM fluorescence, which is monitored at excitation/emission = 490 nm/520 nm. The long wavelength fluorescence of 5-FAM is also less interfered by the autofluorescence of components in biological samples and test compounds. This assay can detect as low as 0.8 ng/mL active IDE.

Specifications

Packaging
Kits components
  • Component A: 5-FAM /QXL® 520 IDE substrate Ex/Em=490 nm/520 nm upon cleavage: 1 mM, 50 µL Component B: 5-FAM fluorescence reference standard, Ex/Em=490 nm/520 nm: 1 mM, 12 µL Component C: Human recombinant IDE: 0.1 mg/mL, 10 µL Component D: Assay Buffer: 25 mL Component E: Inhibitor: 1 M, 10 µL
Chemistry
UniProt number
  • P14735
Properties
Absorbance (nm)
  • 490
Emission (nm)
  • 520
Storage & stability
Storage Conditions
  • Store all kit components at -20°C, except for Component C, which is stored at -80°C. Protect Components A and B from light and moisture. Component D can be stored at room temperature for convenience.
Activity
Application
Biomarker Target
Detection Method
Detection Limit
  • 0.8 ng/ml
Research Area
Sub-category Research Area
Usage
  • Research use
Codes
Code Nacres
  • NA.32

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Citations

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J Endocrinol . 2016 Mar 21 ; 229 221-32 | DOI : 10.1530/JOE-15-0483

  • M.A. Kurauti
  • et al

Hyperinsulinemia caused by dexamethasone treatment is associated with reduced insulin clearance and lower hepatic activity of insulin-degrading enzyme

J Steroid Biochem Mol Biol . 2015 Sep 16 ; 155(Pt A) 1-8 | DOI : 10.1016/j.jsbmb.2015.09.020

  • A.O. Protzek
  • et al

Piceatannol, a natural analogue of resveratrol, effectively reduces beta-amyloid levels via activation of alpha-secretase and matrix metalloproteinase-9

J Func Foods . 2016 May 01 ; 23 124 | DOI : https://doi.org/10.1016/j.jff.2016.02.024

  • C. Boyoung
  • et al

Effect of trichostatin A on gelsolin levels, proteolysis of amyloid precursor protein, and amyloid beta-protein load in the brain of transgenic mouse model of Alzheimer's disease

Curr Alzheimer Res . 2014 Dec 01 ; 11 1002-11 | DOI : 10.2174/1567205011666141107125531

  • W. Yang
  • et al