PCR & qPCR

SilverStar DNA polymerase - 500 U

90.00
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  • Cat.Number : ME-0074-05
  • Availability :
    In stock
  • Shipping conditions : Ice delivery fees must be applied

Quantity

SilverStar is a high-performance thermostable recombinant DNA polymerase, isolated from Thermus aquaticus. It is an extremely robust Taq DNA polymerase that does not require optimization and gives high yields with minimal background consistently from one reaction to another. It is a highly active enzyme that produces excellent results across a wide range of applications such as DNA Array.

- High yields
- Robust PCR
- Highly purified Taq polymerase
- No optimization needed

Activity
The SilverStar has a 5’ ’ 3’ exonuclease activity and also the “extendase” activity allowing TA cloning. The enzyme lacks the proofreading activity 3’ ’ 5’ exonuclease.

Application
The SilverStar will efficiently amplify products up to 10 kb giving high yields and optimal results for application such as array PCR product constructions.

Quality Control
It is specially purified so as to remove traces of DNA that could interfere with some reactions and is free from all non-specific endonucleases, nickases and exonucleases.

Specifications

Packaging
Kits components
  • - One tube (100 µL) of SilverStar DNA polymerase, 500 U (5U/µl) - 2 Tubes (1.5 mL) of 10x reaction buffer (yellow cap): 160mM (NH4 )2SO4 , 670mM Tris-HCl (pH 8.8 at 25°C), 0.1% Tween-20. Without MgCl2 - 2 Tubes (1.5 mL) of MgCl2 50 mM
Quantity & Purity
Concentration
  • 5 U/µl
Storage & stability
Form
  • In solution
Storage Conditions
  • SilverStar DNA Polymerase can be stored at -20°C, in a constant temperature freezer for 12 months (1). SilverStar will remain stable if stored as specified. Repeated freeze-thaw cycles will affect the stability of Reaction Buffer. The Buffer will remain stable at +4°C for a minimum of one month.
Activity
Template type
  • DNA
Unit definition
  • One unit is defined as the amount of enzyme that incorporates 10 nmoles of dNTPs into acid-insoluble form in 30 minutes at 72°C.
Usage
  • Research use
Source
Host
Source / Species
  • Thermus aquaticus
Codes
Code Nacres
  • NA.55

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