Image of a kit SensoLyte Plus 520 MMP-1 Assay Kit Fluorimetric and Enhanced Selectivity
Assay-kits

SensoLyte® Plus 520 MMP-1 Assay Kit Fluorimetric and Enhanced Selectivity - 1 kit

€676.00
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  • Cat.Number : AS-72012
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    In stock
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The SensoLyte® Plus 520 MMP-1 Assay Kit is designed for specifically detecting MMP-1 activity in biological samples which may contain multiple MMPs, such as culture medium, serum, plasma, synovial fluid, and tissue homogenate. A specific anti-MMP-1 monoclonal antibody is used in combination with an MMP fluorogenic substrate, 5-FAM/QXL™520 FRET peptide. The fluorescence signal is monitored at Ex/Em=490 nm/520 nm upon MMP-1-induced cleavage of the FRET substrate.

Specifications

Packaging
Kits components
  • Component A: Microplate coated with monoclonal anti human MMP-1 antibody: 12 X 8 black strips Component B: MMP-1 standard, recombinant human proMMP-1: 10 µg/mL, 10 µL Component C: MMP dilution buffer: 10 mL Component D: 10 X Wash buffer: 50 mL Component E: APMA, 4-aminophenylmercuric acetate (Caution: Contains organic mercury. Dispose according to regulations): 100 mM, 150 µL Component F: MMP-1 substrate 5-FAM/QXL™520 FRET peptide Ex/Em=490 nm/520 nm upon cleavage: 50 µL Component G: Assay buffer: 50 mL Component H: Stop Solution: 10 mL Component I: Adhesive cover strip: 3 sheets
Chemistry
UniProt number
  • P03956
Properties
Absorbance (nm)
  • 490
Emission (nm)
  • 520
Storage & stability
Storage Conditions
  • Store all kit components, except Component B, at -20°C. Store Component B at -80°C. For convenience, Components D, G, H, I can be stored at RT.
Activity
Application
Biomarker Target
Detection Method
Detection Limit
  • 0.3 ng/ml
Research Area
Sub-category Research Area
Usage
  • Research use
Codes
Code Nacres
  • NA.32

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Citations

Pentraxin 3 induces vascular endothelial dysfunction through a P-selectin/Matrix Metalloproteinase-1 pathway

Circulation . 2015 Mar 06 ; 131 1495 | DOI : 10.1161/CIRCULATIONAHA.114.014822

  • A. Carrizzo
  • et al

Oncogenic KIT-containing exosomes increase gastrointestinal stromal tumor cell invasion

Proc Natl Acad Sci USA . 2013 Dec 30 ; 111 711 | DOI : 10.1073/pnas.1310501111

  • S. Atay
  • et al

Increased levels of CCR7 ligands in carotid atherosclerosis: different effects in macrophages and smooth muscle cells

Cardiovasc Res . 2014 Feb 11 ; 102 148 | DOI : 10.1093/cvr/cvu036

  • B. Halvorsen
  • et al

Effect of thrombin on human amnion mesenchymal cells, mouse fetal membranes, and preterm birth

J Biol Chem . 2014 Mar 20 ; 289 13295 | DOI : 10.1074/jbc.M114.550541

  • H. Mogami
  • et al

IL-1β-induced matrix metalloproteinase-13 is activated by a disintegrin and metalloprotease-28-regulated proliferation of human osteoblast-like cells

Exp Cell Res . 2014 Mar 05 ; 323 165 | DOI : 10.1016/j.yexcr.2014.02.018

  • N. Ozeki
  • et al

Fetal fibronectin signaling induces matrix metalloproteases and cyclooxygenase-2 (COX-2) in amnion cells and preterm birth in mice

J Biol Chem . 2012 Nov 26 ; 288 1953 | DOI : 10.1074/jbc.M112.424366

  • H. Mogami
  • et al

The PPARγ Agonist Rosiglitazone Is Antifibrotic for Scleroderma Lung Fibroblasts: Mechanisms of Action and Differential Racial Effects.

Pulm Med . 2011 Nov 01 ; 2012 545172 | DOI : 10.1155/2012/545172

  • G.S. Bogatkevich
  • et al

Physiological loading of joints prevents cartilage degradation through CITED2

FASEB J . 2010 Sep 08 ; 25 182 | DOI : 10.1096/fj.10-164277

  • D. Leong
  • et al

Epidermal growth factor-induced matrix metalloproteinase-1 expression is negatively regulated by p38 MAPK in human skin fibroblasts

J Dermatol Sci . 2011 Aug 04 ; 64 134 | DOI : 10.1016/j.jdermsci.2011.07.002

  • C. Park
  • et al

Adiponectin activation of AMPK disrupts leptin-mediated hepatic fibrosis via suppressors of cytokine signaling (SOCS-3)

J Cell Biochem . 2010 Aug 01 ; 110 1195 | DOI : 10.1002/jcb.22634

  • J. Handy
  • et al

Collagenase expression and activity in the stromal cells from giant cell tumour of bone

Bone . 2009 Feb 12 ; 44 865 | DOI : 10.1016/j.bone.2009.01.393

  • R.W. Cowan
  • et al

Iron sensitizes keratinocytes and fibroblasts to UVA-mediated matrix metalloproteinase-1 through TNF-α and ERK activation

Exp Dermatol . 2011 Mar 01 ; 20 249 | DOI : 10.1111/j.1600-0625.2010.01152.x

  • J. Jian
  • et al

Proteolytic activity in wound fluids and tissues derived from chronic venous leg ulcers

Wound Repair Regen. . 2009 Nov 01 ; 17 832 | DOI : 10.1111/j.1524-475X.2009.00547.x

  • A. Moor
  • et al

Interstitial flow promotes vascular fibroblast, myofibroblast, and smooth muscle cell motility in 3-D collagen I via upregulation of MMP-1

Am J Physiol Heart Circ Physiol . 2009 May 22 ; 297 H1225 | DOI : 10.1152/ajpheart.00369.2009

  • Z-D. Shi
  • et al

Fluid shear stress regulates the invasive potential of glioma cells via modulation of migatory activity and matrix metalloproteinase expression

PLoS One . 2011 May 26 ; 6 e20348 | DOI : 10.1371/journal.pone.0020348

  • H. Qazi
  • et al