We use cookies to offer you the best experience on our site. You can find out more about the cookies we use or disable them in the Cookie settings
Custom manufacturing
Custom Gene Synthesis
Custom Genes are synthetic genes optimized to face any hindrance encountered with classical PCR amplified nucleotide fragments. It considerably reduces time spending on oligonucleotides design, cloning process and sequencing.
Why choosing custom genes?
De novo synthesis of DNA considerably improve the protein expression yield and quality.
They guarantee the absence of undesired mutation or single nucleotide polymorphism (SNP), which may influence downstream applications.
%
Guaranteed sequence
Custom genes benefits
- From simple gene to highly complex sequence
- Allow gene optimization
- Fast and simple
Base pairs
Length up to 50 kbp
to 20
Working Days
Depending on the sequence length
µg of plasmid DNA
Carrying your Custom Gene(s)
Applications of synthetic genes
- Gene variants and SNPs
- RNA optimization
- Codon optimization
- Synthetic Biology
- cDNA design
- Microarray-ready cDNA
- Recombinant antibodies
- DNA vaccines and vectors
Gene synthesis features
Your gene
- Gene length up to 50 000 bp
- GC rich content compatible
- Repeated sequences are not an issue
- Adding or removing restriction sites
- Adding or removing specific constraints
Optimization
Optimization of sequences can be applied to your gene to ensure the best regulation:
- TATA boxes
- SD sequence
- Terminal signal
- GC content
- Cryptic splicing sites
- Negative CpG islands
- Codon usage bias
- mRNA secondary structure
- Premature PolyA sites
- RNA instability motif
Vector
- Free cloning into pUC57 (by default)
- Cloning in your own vector or other vectors such as pcDNA and pET types available on request
Quality
Trusted quality ensured:
- 100 % sequence of your Custom Gene(s)
- Size of the inserted fragment
- Flanking regions of the chosen vector
- DNA quality and quantity
Delivery
You will receive:
- Custom Gene(s) ≥ 2000 bp delivered within 15-20 WDays
- Delivery in 8-10 WDays Option available for genes ≤ 800 bp without complex sequences
- 4 µg of lyophilized plasmid DNA carrying your Custom Gene(s)
- A printout of the sequence alignment confirmed by sequencing
- A plasmid map to locate the flanking regions and restriction sites
- General information and instructions to directly use your Custom Gene(s)
- Quality Assurance Certificate
Gene Optimization Service
Increase up to 20 times your protein production level in E. coli!
Expression of a native gene in a heterologous host often involves sequence preference incompatibility, which may result in low or no expression. At Eurogentec, we offer gene optimization as a free service.
Generate gene sequences with the highest possible levels of productivity
Available in any given expression system, our method takes into account a variety of critical factors involved in different stages of protein expression, such as codon adaptability, mRNA structure, GC content, repetitive sequences and various others cis-elements in transcription and translation.