We use cookies to offer you the best experience on our site. You can find out more about the cookies we use or disable them in the Cookie settings
Endpoint PCR
IVD Polymerases
Diamond Taq® family polymerases show very good sensitivity and exceptional reproducibility. As highly thermostable enzymes, they offer an ideal solution for Molecular Diagnostic and demanding Research PCR & qPCR.
These Polymerases are purified from recombinant Escherichia bacteria containing the Thermus aquaticus DNA polymerase gene. This thermophilic eubacterium strain lacks TaqI restriction endonuclease and catalyzes 5’> 3’ synthesis of DNA with no detectable 3’> 5’ exonuclease activity. The enzyme has the 3’ extendase activity allowing TA cloning./p>
Ultra pure Diamond Taq® Benefits
Our IVD enzymes are highly suited for Diagnostic kits, Lab Services (with ASRs) and demanding Research
Exceptional Reproducibility
Stringent process and QC ensuring lot-to-lot & results reproducibility
Full
traceability
Quality Management System fully compliant to ISO 13 485 Medical Device standards and FDA’s Quality System Regulations
Customized fill
& finish
Cost-effective tailored solution
Ultra-low residual DNA content
<1 fg of genomic E. coli DNA / Taq Unit Typically < 0.01 fg or 0.002 E. coli genome copies /Taq Unit
Purity
Highly pure (> 98 %) and ultra-low bioburden (≤ 10 CFU/ml). Typically = 0 CFU/ml
Sensitivity
Amplification of DNA templates even at very low concentrations
How to select a polymerase?
| PCR | qPCR | Yield | Sensitivity Low Copy Template | Specificity | Difficult Template (GC & AT rich) | IP Burden | Free Sample | |
|---|---|---|---|---|---|---|---|---|
| Hot Diamond Taq® Short activation time |
+++ | ++ | +++ | +++ | +++ | +++ | + | TAQ-I032-100 |
| HGS Diamond Taq® Heat stability improved |
+++ | +++ | +++ | +++ | +++ | ++ | +++ | TAQ-I010-100 |
| Diamond Taq® | +++ | + | ++ | ++ | ++ | + | + | TAQ-I020-100 |
Hot Diamond Taq®
The Hot Diamond Taq® represents a completely new “HotStart Concept”. Neither accomplished through chemical modification nor a blocking antibody but a proprietary agent. This polymerase needs a really short activation time (20s are sufficient) and remains compatible with all existing protocols.
Fig: Hot Diamond Taq® was evaluated using from 20 seconds to 10 minutes as activation time for its ability to amplify 1 and 5 pg of λDNA. PCR products were analyzed by gel electrophoresis.
Traceability
All these enzymes, shipped on dry ice, follow the IVD traceability
at each step of the process, a Tracking number is sent to customer the day of the shipment.
Specifications
| Parameters | Specifications |
|---|---|
| Appearance* | Colorless solution |
| Identity (SDS-PAGE) | MW approx. 95 kDa |
| Volume activity | ≥ 5U/µl |
| Purity (SDS-PAGE) | > 98% |
| Performance test: PCR-λ DNA* | 0.5 kb fragment positive down to 5 pg |
| Performance test: PCR – 18S DNA* | 0.1 kb fragment positive down to 10 pg |
| Ribonucleases (up to 10U, 1h, 37°C) | Not detectable |
| Endonucleases, Exonucleases, Nicking activity (up to 30U, 16h, 65°C) | Not detectable |
| E.coli residual DNA | < 1 fg/Taq Unit |
| Bioburden* | ≤ 10 CFU/ml |
| Stability* | 24 months (at –20°C) from date of manufacture |
| Animal-derived additives* | None |
| HotStart | No detectable amplification without activation |
| Performance test PCR-Numb DNA (Hot Diamond Taq®) | 0.3 kb fragment positive down to 10 pg |