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Low ROX qPCR kits for probe assays
We recommend a low ROX qPCR kit according to your thermocycler selection.
Step 2
Select your format
2x Master Mix
- Ready-to-use all-in-one qPCR reagent
- Maximum reproducibility
- Minimal contamination risk
5x Master Mix
- Ready-to-use all-in-one qPCR reagent
- Maximum reproducibility
- Minimal contamination risk
5X MasterMixes allow to template volume optimization thanks to the high sensitivity of TakyonTM kits
Components in separate tubes (Core Kits)
Individually optimized assay component
- Cost effective
- Maximum flexibility
- Maximum assay performance through component concentration optimization
Select your options
2x Master Mix
| Clear | Blue | |
|---|---|---|
| With carry-over prevention | Clear UNG mix | Clear UNG mix + Blue additive |
| Without carry-over prevention | Clear dTTP mix | Blue dTTP mix |
5x Master Mix
Classical
| Clear | Blue | |
|---|---|---|
| With carry-over prevention | Clear dTTP mix + dUTP/UNG additive | Clear dTTP mix + dUTP/UNG additive + Blue additive |
| Without carry-over prevention | Clear dTTP mix | Clear dTTP mix + Blue additive |
Core kits
| Clear | Blue | |
|---|---|---|
| Carry-over prevention (UNG additive included) | Clear | Clear + Blue additive |
Options for your qPCR kit
Visualization
The inert blue dye included into Blue kits allows you to visualize dispensing errors at a glance and avoid aberrant results before they occur. This is particularly true when using white plates, known for their superior performances.
Blue additive is available to convert clear mixes.
Carry-over prevention
Any trace of DNA molecule from previous reaction containing dUTP will be removed by the action of the UNG.
- UNG kits contain dUTP and UNG.
- dTTP kits can be converted by adding dUTP/UNG additive.
Assay automation
The low viscosity HTP Takyon™ kits are perfectly suited for assay set-up automation.