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ANTIBODY PRODUCTION SERVICES
Standard & Fast Polyclonal Antibody Programs
We produce custom polyclonal antibodies in various host species from antigens provided by our customers or synthesized at Eurogentec. We offer polyclonal antibody preparation from our standard or fast immunization programs. We can also customize the program according to the needs of our customers.
Antigens for custom polyclonal antibody generation
We produce polyclonal antibodies with various types of antigen including peptides, proteins, or complex samples. We use antigens provided by our customers or we synthesize antigen peptides.
You provide the antigen
You can provide us with your protein, peptide, cell extract, or complex sample. In this case, we recommend that you consult the how to provide my antigen section.
Injecting a complex sample (protein, cell extract…) will result in rich polyclonal serum with a broad variety of different antibodies. Some of them will potentially target conformational epitopes.
We synthesize the antigen
Eurogentec designs and synthesizes 15-25 mg of one peptide (≥70% purity, 10-16 aa). Injecting one peptide will result in a serum containing a limited set of different polyclonal antibodies targeting linear -but not conformational- epitopes.
Double X Option
Eurogentec designs and synthesizes two peptides (15-25 mg each, ≥70% purity, 10-16 aa).
The DX programs use two peptides to increase the chances of success. These peptides correspond to spatially distinct regions of the target protein surface. This increases the opportunity for the resultant polyclonal to recognize the target protein in its natural environment. Each host receives a combination of the two peptides.
The customer then tests the serum from the immunized host(s) in its own assay. If the result is conclusive, we recommend performing a small-scale antibody affinity purification with each peptide independently. The customer tests the purified antibodies to determine whether one or both peptides triggered the production of target-specific antibodies.
Anti-Post Translational Modification (PTM)
Eurogentec designs and synthesizes two peptides: one with the PTM (15-20 mg, >70% purity, 10-16 aa) and one unmodified.
Select the best host
Selecting a host for the production of polyclonal antibodies depends on various factors.
Antigen quantity vs serum volume
The quantity of antigen requested for the immunization depends on the host species and the expected volume of serum.