Production process

We immunize Llama’s or Alpaca’s to provide blood samples containing both classical and heavy-chain only antibodies and offer VHH development and production in collaboration with QVQ.

The production process and Options are detailed below.

1.

Immunization

At Eurogentec, llama or alpaca immunizations are conducted using customized protocols and antigens to produce heavy chain only antibodies.

Serum samples are available for testing or for IgG’s isolation.

 

5 – 10 weeks*

Deliverables

The program can be stopped at this step and you receive unpurified pAb (blood or serum).

 

*Average time

2.

Lymphocytes isolation & mRNA extraction

Blood lymphocytes necessary for VHH production are poorly stable.

So we provide the service for lymphocyte isolation from a blood sample (+/- 250 mL) and the RNA extraction.

 

1 Day*

Deliverables

The program can be stopped at this step and you receive mRNA.

 

*Average time

3.

VHH screening & production

At QVQ, VHH phage display libraries are generated to screen for high affinity monoclonal VHH.

 

8 weeks*

Deliverables

  • Step 1 : Two libraries with size >107 different clones and > 90% insert.
  • Step 2 : The sequences of at least 6 binders from 2 families**.
  • Step 3 : 0.5 mg protein of the lead clones produced in E.coli and purified (>80%).

*Average time

** When the clone diversity allows

Labeling

VHH fragments can be custom labeled for a wide range of applications including immunofluorescence, FACS analysis, in vivo imaging, PET imaging, MRI imaging, IHC and electron microscopy.

Large scale & GMP production

We can produce large quantities of VHH antibody fragment in yeast for animal experiments or crystallography up to GMP grade for microdosing studies in human.

Optimization

The sequence of the VHH fragments can be optimized for production, physical and “in product” stability and various types of clinical use.

Formatting

VHH antibody fragments can be generated in bivalent, bispecific or custom made formats.

VHH antibody fragments

These are the smallest naturally occurring antibody binding domains presents many advantages and hence are considered as very promising candidates for novel therapy.

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Anti-llama secondary antibodies

We provide labeled (biotin, R-PE or custom labeled) and unlabeled secondary antibodies to target llama IgG1 or IgG2/3 immunoglobulins with high precision and sensitivity.

We also offer a unique top-quality secondary antibody targeting VHH’s. This rabbit polyclonal is broad VHH-specific, as it binds to and allows the detection of VHHs from different germline sequence families.

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Confidentiality

Eurogentec and QVQ ensure that your project and your data will be handled under full confidentiality and will never be shared with external parties. Non-disclosure agreements can be executed.